The accident occurred at around 4pm on Tuesday when a car got stuck between the barriers on a railway crossing point, Bern police said in a statement.
It was then hit by a train travelling from Interlaken Ost station.
The two occupants of the car managed to leave their vehicle before the crash, and no one on the train was injured, said police.
The 200 train passengers were evacuated and the line between Interlaken West and Ost was interrupted.
An investigation has been opened to determine the cause of the accident, said police.
There have been several train accidents in Switzerland this year.
In May a train travelling from Italy derailed outside Lucerne station, injuring seven people.
A week later another train derailed near Bern; no one was injured.
Last year a man was killed when his vehicle ended up on a railway line and was struck by an oncoming train.